R&D Systems/ Perseus Proteomics Inc
anti-human COUP-TFII
PP-H7147-00
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Browse Similar Products: COUP-TFII Immunofluorescence Antibodies
This antibody was used to detect venous endothelial cells. We differentiated iPSCs to endothelial cells and cultured these endothelial cells in culture conditions to promote venous differentiation. COUP-TFII is a venous marker and this antibody was helpful in identifying positive cells by immunostaining.
Immunofluorescence
Endothelial cells derived from human ESCs
1:1000, over night under refrigeration
Rabbit serum in PBST
1:2000, 1hr at room temp, Rabbit anti-mouse secondary antibody
None
Fluorescence
This antibody gave very clean and clear nuclear staining of endothelial cells positive for the venous marker.
29583085
N/A
Very easy to use and optimize
This is a very good antibody for identifying venous endothelial cells.