ATF-4 Antibody

April 13, 2018

Center for Translational Biomedical Research
UNC Greensboro
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Company:

Cell Signaling Technology

Product Name:

ATF-4 Antibody

Catalog Number:

11815

This antibody was used in a study that investigated the role of ALOX15 and its product 13-HODE in alcoholic fatty liver. It has been published in Sci Rep in 2017. The antibody reviewed here gave the best results in western blot using mouse liver.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse liver

Primary Incubation

1:1000, 10 hour at 4 degree

Blocking Agent

5% milk

Secondary Incubation

1:5000, 1 hour at 25 degree

Tertiary Incubation

NA

Detection

ECL

Results Summary

Alcohol feeding significantly induced ATF-4 expression, which was suppressed by ALOX15 knockout.

DOI or PMID #

28827690

Additional Notes

N/A

Image Gallery

Summary

The Good

Very specific

The Bad

N/A

The Bottom Line

Super good for western blot using mouse liver.

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