ATF-4 (D4B8) Rabbit mAb 11815S from Cell Signaling Technology

Product Specs
ItemATF-4 (D4B8) Rabbit mAb
CompanyCell Signaling Technology
Price $347.00 Inquire
Catalog Number11815S
Quantity100 µl
ApplicationsW, IP, IF-IC, ChIP, ChIP-seq, C&R, C&T
ReactivityH, M, R
TargetATF-4
IsotypeIgG
HostRabbit
NCBI Full Gene Nameactivating transcription factor 4
NCBI Gene AliasesCREB-2, CREB2, TAXREB67, TXREB
CloneD4B8
Molecular Weight49
ClonalityMonoclonal
Research AreaMetabolism

Questions and Answers

Can this antibody be used to study the unfolded protein response in cancer cells?

Yes, the ATF-4 (D4B8) Rabbit mAb has been utilized in studies investigating the unfolded protein response, particularly in the context of endoplasmic reticulum stress. For instance, it was used to assess ATF4 expression in breast cancer cells treated with a novel herbal extract that induces ER stress, highlighting its role in apoptosis and the unfolded protein response pathway ().

Is this antibody effective for Western blotting applications?

The ATF-4 (D4B8) Rabbit mAb has been shown to work effectively in Western blotting applications. It has been cited in multiple studies where it was used to detect ATF4 protein levels in various experimental setups, including those involving xenograft models and cancer cell lines (, ).

What species can this antibody react with?

This antibody is reactive with multiple species, including human (Homo sapiens), mouse (Mus musculus), and rat (Rattus norvegicus). This broad reactivity makes it suitable for various experimental models in cell biology research.

Has this antibody been used in any specific cancer research contexts?

Yes, the ATF-4 (D4B8) Rabbit mAb has been referenced in studies related to cancer, including its role in enhancing the efficacy of chemotherapy in neuroblastoma models and its involvement in the stress response mechanisms in malignant pleural mesothelioma (, ).

Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) A microRNA generated via lysosomal processing of ribosomal RNA suppresses proinflammatory responsesLife Science AllianceJuly 1, 2026Dan Michael, Ester Feldmesser, K Shanmugha Rajan, Yoav Lubelsky, Anat Bashan, Alexandros Damalas, Shiri Ben Zvi, Aya Friedberg, Netta Eitan, Shachar Erez, et al.(Protran BA-83; Whatman). Membranes were reacted with anti-GAPDH (MAB-374; Millipore) anti-ATF4 (D4B8, 11815; Cell Signaling), and IRE1 (14C10, # 3294; Cell Signaling) primary antibodies diluted in 2% BSA in PBST

(2) Damage to newly synthesized proteins is a major cause of Cd(II) toxicity counteracted by proteasomes and integrated stress response in human cellsChemico-Biological InteractionsJune 1, 2026Giorgiana Madalina Ursu, Anatoly Zhitkovich(Cell Signaling, 58395), phospho-eIF2α (Cell Signaling, 9721), eIF2α (Cell Signaling, 5324), ATF4 (Cell Signaling, 11815), SOD1 (Cell Signaling, 2770), fibrillarin (Cell Signaling, 2639), histone H3 (Cell Signaling, 4499),

(3) Overcoming ADC resistance in advanced colorectal cancer by dual targeting of TROP2 and PERK to suppress Wnt/β-catenin signalingCell Reports MedicineMay 19, 2026Jie Liu, Mei Li, Jianming Huang, Yujie Xia, Guanfeng Jiang, Hong’an Zhang, Jiaxin Yang, Mingfeng Jiang, Yalan Liu, Yilun Luo, et al.anti-Phospho-eIF2α,Proteintech,Cat#68023-1-Ig; RRID:AB_2918767 Rabbit monoclonal anti-ATF4,Cell Signaling Technology,Cat#11815S; RRID:AB_2616025 Rabbit monoclonal anti-β-Catenin,Proteintech,Cat#51067-2-AP; RRID:AB_2086128 Rabbit

(4) Neuromuscular junction innervation and motor function are preserved by restoring muscarinic signaling in perisynaptic glia in ALSiScienceMay 15, 2026Elsa Tremblay, Danielle Arbour, Joanne Vallée, Roberta Piovesana, Geneviève Vallières, Emine Mechichi, Richard Robitaillemin with TBS-0.1% Tween 20 buffer, membranes were incubated with the primary antibody (ATF4, 1:1000, #11815, Cell Signaling, overnight; diluted in TBS-Tween Solution with 5% Bovine Serum Albumin (BSA)). Membranes

(5) Bufalin Suppresses Pancreatic Ductal Adenocarcinoma Through ER Stress-Ferroptosis Crosstalk Associated with IP3R-Linked Ca2+ Dysregulation and ATF3/SLC7A11 RegulationInt J Mol SciMay 14, 2026Pei-Wen Yang, Xin Li, Wai-Mei Si, Yuan Zhang, Xiang-Yu Kong, Xin-Yi Xu, Xiao-Yan Zhu, Zhen ChenRatio ATF3,"Abcam (Waltham, MA, USA)",ab254268,1:1000 ATF4,"Cell Signaling Technology (Danvers, MA, USA)",11815,1:1000 p-PERK,"Abconal Biotech (Wuhan, China)",AP1501,1:1000 PERK,"Abconal Biotech Wuhan, China)",A18196,1:1000 phospho-eIF2α

(6) A High-Molecular-Weight Fraction of Planarian Mucus Triggers UPR-Linked Cell Death Pathway in Human Bronchioalveolar Carcinoma Cell Line NCI-H358Int J Mol SciMay 12, 2026Gaetana Gambino, Gemma Marcelli, Paola Iacopetti, Laura Benvenuti, Chiara Bertini, Lucia Giambastiani, Luisa Pozzo, Alessandra Salvetti, Leonardo Rossimilk (Merck KGaA, Darmstadt, Germany) in TBST, and then incubated overnight at 4 °C with anti-ATF4 (#11815, Cell Signaling, Danvers, MA, USA; rabbit monoclonal antibody, 1:1000 in 5% BSA in TBST) or anti-ATF6

(7) Impaired glycosylation promotes rapid transition to hepatocellular carcinoma in model of diet induced steatotic liver diseaseJournal of Clinical InvestigationMay 1, 2026Abhishek K. Singh, Balkrishna Chaube, Kathryn M. Citrin, Joseph W.M. Fowler, Sungwoon Lee, Jonatas Catarino, James Knight, Sarah C. Lowery, Sonal Shree, Keira E. Mahoney, et al.onto nitrocellulose membranes and probed with various antibodies, such as anti-ATF4 (Cell Signaling, 11815, 1:1,000), anti-NgBR (Abcam, Ab168351), anti–β-actin (Sigma-Aldrich, A1978), and anti-HSP90 (F-8) (Santa Cruz Biotechnology

(8) Melatonin Partially Attenuates Oxycodone-Induced Placental Stress Signaling and Fetal Brain Apoptosis in a Sex-Specific MannerbioRxivMay 1, 2026Isaac O Adediji, Kajal Kamra, Hager M Kowash, P Nouri Nouri Mousa, Christianah O Aloba, Victoria L Schaal, John S Davis, Eric S Peeples, Gurudutt N Pendyala, Lynda K HarrisCST#3183), CHOP (1:500, Cell Signaling Technology, CST#2895), ATF4 (1:500, Cell Signaling Technology, CST#11815), TLR4 (1:500, Abcam, #ab22048), IL-1β (1:500, Abcam, #ab254360), TNF-α (1:500, Abcam, #ab215188), cleaved

(9) RNA-binding protein ELAVL1 modulates WDR36 to inhibit p53 pathway and reduce calcium overload in retinal cells under acute pressure elevationOpen Life SciApril 20, 2026QingFeng Meng, BaoLi Ning, WenXiong Chen, JingJing Zhang, Jun FuCell Signaling Technology), CHOP (#2895, Cell Signaling Technology), GRP78/BiP (#3177, Cell Signaling Technology), ATF4 (#11815, Cell Signaling Technology), p-PERK (Thr980) (#3179, Cell Signaling Technology), p-IRE1α (Ser724) (ab124945,

(10) Activation of GABBR1 by New Ligand Valerate Transcriptionally Regulates ATF4-NRF2-CXCL13 Axis Mediating CD8+ T Cell Anti-Tumor ImmunityInt J Biol SciApril 16, 2026Zhiran Zhai, Jumin Huang, Jiayue Qiu, Yuwei Wang, Jin Liao, Chun Xie, Jiahui Xu, Zebo Jiang, Yajia Xie, Wenkang Ren, et al.anti-GABBR1 (#ab238130, Abcam, 1:800), anti-NRF2 (#12721, Cell Signaling Technology, 1:1000), anti-ATF-4 (#11815, Cell Signaling Technology, 1:1000), anti-NF-κB1 (p65) (#8242, Cell Signaling Technology, 1:1000), anti-NF-κB2 (p100/p52

(11) Neuronal SEL1L-HRD1 ER-associated degradation is essential for motor function and survival in miceJournal of Clinical InvestigationApril 15, 2026Mauricio Torres, You Lu, Brent Pederson, Hui Wang, Anna Gretzinger, Liangguang L. Lin, Jiwon Hwang, Xinxin Chen, Alan C. Rupp, Abigail J. Tomlinson, et al.1:1,000), anti-eIF2α (Cell Signaling Technology, 9722, 1:1,000), and anti-ATF4 (Cell Signaling Technology, 11815, 1:1,000). After washing with TBST, membranes were incubated with HRP-conjugated secondary antibodies

(12) MEK-dependent bioenergetic demand drives terminal CD8 + T cell exhaustionbioRxivApril 12, 2026Tanmana Mitra, Jahan Rahman, Madeline Hwee, Ruben Jose Jesus Faustino Ramos, Hui Liu, Travis Hartman, Justin Cross, Miguel de Jesus, Morgan Huse, Valerie Longo, et al.Protein (CST #2708), p-S6 Ribosomal Protein (Ser240/244) (CST #9234), CHOP (CST #2895), ATF-4 (CST #11815), BiP (CST #3177), p-p38 MAPK (Thr180/Tyr182) (CST #4511), p38 MAPK (CST #9228), RNA Polymerase II CTD

(13) Antigenic cancer persister cells survive direct T cell attackbioRxivApril 12, 2026Michael X. Wang, Brandon E. Mauch, August F. Williams, Tania Barazande-Pour, Filipe Araujo Araujo Hoffmann, Sophie H. Harris, Cooper P. Lathrop, Claire E. Turkal, Bryan S. Yung, Michelle H. Paw, et al.#9239, 1:1000), BAX (CST #5023, 1:1000), BAK (CST #12105, 1:1000), ATF3 (CST #33593, 1:1000), ATF4 (CST #11815, 1:1000), NRF2 (CST #12721, 1:1000), SLC7A11 (CST #12691, 1:1000), H3K14ac (CST #7627, 1:1000), H3K4me3

(14) Serglycin Cooperates with the Unfolded Protein Response Pathway and Inflammation to Drive Glioblastoma Cell SurvivalCellsApril 9, 2026Eleftherios N. Athanasopoulos, Chrysostomi Gialeli, Angeliki Natsiou, Dimitra Manou, Vassiliki T. Labropoulou, Achilleas D. Theocharistotal-Akt (Cell Signaling Technology Inc., #9272, dilution 1:1000), ATF4 (Cell Signaling Technology Inc., #11815, dilution 1:1000), ATF6 (Cell Signaling Technology Inc., #65880, dilution 1:1000), BiP (Cell Signaling Technology

(15) Stress adaptation pathways and HA–CD44 signaling maintain the survival of pancreatic cancer cells with centrosome amplificationCell Communication and SignalingApril 7, 2026Selahattin Can Ozcan, Evrim Goksel, Batuhan Mert Kalkan, Enes Cicek, Beste Kanevetci, Ceyda AcilanHistone H3 (Cell Signaling, 4499, 1:2000), NRF2 (Cell Signaling, 12721, 1:1000), ATF4 (Cell Signaling, 11815, 1:1000), ATF6 (Cell Signaling, 65880, 1:1000), IRE1\documentclass[12pt]{minimal} \usepackage{amsmath}

(16) Toxoplasma gondii effector GRA35 mediates neuronal damage via ER stress and mitochondria-associated apoptosisVirulenceApril 6, 2026Jie Wang, Ying Chen, Nan Zhou, Fangmin Li, Niuniu Dai, Zhiang Chen, Shutong Liu, Ran An, Lijian Chen, Jian Du3179S), p-eIF2α rabbit polyclonal antibody(Catalog #: 9721S), ATF4 rabbit polyclonal antibody(Catalog #: 11815S), GRP78 rabbit polyclonal antibody(Catalog #: 3177S), CHOP rabbit polyclonal antibody (Catalog #: 5554S)

(17) Posttranscriptional reprogramming controls MASLD progression through chronic ER stress adaptationSci AdvApril 3, 2026Eulalia Belloc, Vittorio Calderone, Salvador Naranjo-Suarez, Lidia Mateo, Judit Martin, Florencia Malizia, Annarita Sibilio, Veronica Chanes, Marta Ramirez-Pedraza, M. Eugenia Delgado, et al.experiment, protein extraction was done in RIPA buffer. The expression of ATF4 (Cell Signaling Technology, 11815; 1:1000) and CHOP (Cell Signaling Technology, CST5554T; 1:500) was analyzed by Western blot. Vinculin

(18) Expansion of outer cortical CUX2 neurons requires adaptations for DNA repairNatureApril 1, 2026Wenlong Xia, Laura Morcom, Zhaoyang Xu, I-Ling Lu, Qing Wang, Kimberly K. Hoi, Mingming Wei, Keying Zhu, Gregory Jordan, Xiao-Yan Tang, et al.detected with antibody 10209-2-AP (Proteintech) at 1:500 dilution; and ATF4 was detected with antibody 11815 (Cell Signaling Technology) at 1:500 dilution. All secondary antibodies used for immunoblotting were

(19) METTL18 ensures pancreatic function by maintaining proper translation and proteostasisMolecular MetabolismApril 1, 2026Tadahiro Shimazu, Megumi Gowa, Ayane Kataoka, Takehiro Suzuki, Kotaro Tomuro, Yuichi Shichino, Naoshi Dohmae, Shintaro Iwasaki, Yoichi Shinkai(cat#15850-1-AP), and anti-PERK/EIF2AK3 (cat#24390-1-AP) (Proteintech Group); anti-IRE1 (cat#3294), anti-ATF4 (cat#11815), and anti-eIF5A (cat#20765) (Cell Signaling Technology); anti-phospho-IRE1 (cat#ab48187), anti-phospho-EIF2A

(20) ORMDL Proteins Turnover via Proteasome and Autophagy Is Cell-Type Dependent and Tied to Ceramide HomeostasisFASEB JMarch 31, 2026Michal Mrkacek, Magda Tumova, Alex Puskasu, Pavol Utekal, Marek Vrbacky, Ladislav Kuchar, Petr Draber, Viktor Bugajevanti‐phospho‐AKTSer473 (Cat# 4060, RRID:AB_2315049), anti‐LC3A/B (Cat# 12741, RRID:AB_2617131), and anti‐ATF4 (Cat# 11815, RRID:AB_2616025) from Cell Signaling Technology, Danvers, MA, USA; anti‐p62/Sequestosome 1 (p62/SQSTM1)

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4/13/2018ATF-4 Antibody Wenliang ZhangThis antibody was used in a study that investigated the role of ALOX15 and its product 13-HODE in alcoholic fatty liver. It has been published in Sci Rep in 2017. The antibody reviewed here gave the ... Read Review

ATF-4 (D4B8) Rabbit mAb

ATF-4 (D4B8) Rabbit mAb from Cell Signaling Technology

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ATF-4 (D4B8) Rabbit mAb from Cell Signaling Technology

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