ThermoFisher Scientific
SYBR™ Safe DNA Gel Stain
S33102
Info:Supplier Page View Product Specs View Product
I have used ThermoFisher's SYBR Safe DNA gel stain to visualize my PCR products used for cloning. PCR products visualized with this stain and gel purified and used for cloning gave improved cloning efficiency with no mutations in the final cloning constructs. SYBR Safe DNA gel stain used was less toxic and safer to use compared to ethidium bromide.
DNA visualization in agarose gels
5 microliter of DNA per well in 1 percent agarose gel stained with SYBR Safe DNA gel stain
Protocol very simple. Prepare 1% agarose gel in 1X strength TAE buffer. Once agarose gets melted, cool it to 60 degree C. Add SYBR Safe DNA stain to the agarose at 1 in 10,000 dilution. Allow the agarose to set at room temperature (solidification). Once agarose was set, place the agarose in a gel tank and load the wells with DNA marker/ladder and your samples. Perform electrophoresis at required electric volts. Then visualize the gel in BioRad gel imager.
Protect the dye from exposure to light when it is stored at room temperature.
Very sensitive DNA gel stain. Clear DNA bands were even visualized in Blue Light LED transilluminator.
N/A
None
Highly sensitive and more safer to handle compared to ethidium bromide stain.
Highly recommended for molecular biology laboratory.