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Author Profile
Triveni Mahendrappa
Research Assistant
University of Birmingham
Articles by Triveni Mahendrappa
Assay Kit/Reagent User Review
Restriction Cloning Using SpeI-HF
May 08, 2018
I have used SpeI-HF restriction enzyme from NEB to digest the plasmid called pSSPgl of a size 24 Kb. Digestion resulted in 23 and ...
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Assay Kit/Reagent User Review
Transformation Of Expression Plasmid Constructs
May 07, 2018
I have constructed a expression plasmid called pACYC-cadA and transformed into to Invitrogen's One Shot Top10 chemically competent...
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Assay Kit/Reagent User Review
Diagnostic Restriction Mapping The Plasmid Construct With AatII
May 04, 2018
I have constructed a plasmid called pTS30-flaB of a size 7.13 kb. The plasmid was prepped from the transformed E. coli cells and ...
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Assay Kit/Reagent User Review
Checking Adaptor Ligated Whole Genomic DNA Sequencing Library Fragment Size
May 03, 2018
I have sheared whole genomic DNA sequencing library to 400 bp and ligated them with Illumina sequencing adaptors. After ligation ...
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Assay Kit/Reagent User Review
Diagnostic Restriction Mapping Of The Plasmid Construct With XhoI
May 02, 2018
I have designed and constructed a plasmid, pET20-cadA of size 6130 bp. After transforming this plasmid into E. coli BL21 cells, ...
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Assay Kit/Reagent User Review
Diagnostic Restriction Mapping Cloned Plasmid Constructs From E.Coli Cells Using NotI-HF
May 01, 2018
I have constructed a plasmid called pET-evgS of size 7.12 kb. This plasmid was transformed into E. coli DH5 alpha cells and ...
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Assay Kit/Reagent User Review
Diagnostic Restriction Mapping Of The Plasmid Construct With NcoI-HF
April 30, 2018
I have constructed a plasmid construct called pCU190_dgp of a size approximately 19 kb by Gibson assembly method. This plasmid ...
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Assay Kit/Reagent User Review
Diagnostic Restriction Mapping Of The Plasmid Construct With PvuII Enzyme
April 27, 2018
I have constructed a plasmid called pUC19_Pgl2 of a size approximately 6.95 kb and transformed it into E.coli cells. After ...
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Assay Kit/Reagent User Review
Amplification Of High Quality DNA Fragment With High Fidelity Using Pwo DNA Polymerase
April 26, 2018
I have used Pwo DNA polymerase for amplification of 6 kb DNA fragment with high fidelity for cloning into expression plasmid. PCR ...
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Assay Kit/Reagent User Review
Diagnostic Restriction Mapping Of The Plasmid Construct With AflII Enzyme
April 25, 2018
I have constructed a plasmid called pUC19_atpA of size 5113 bp. These constructed were transformed into E. coli DH5 alpha cells. ...
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Assay Kit/Reagent User Review
Diagnostic Restriction Mapping Of Plasmid Construct With BglII Restriction Enzme
April 24, 2018
I have constructed a plasmid called pCVD442_operon B of a size 18992 bp size. This plasmid was prepped from carrier E. coli ...
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Assay Kit/Reagent User Review
Diagnostic Restriction Mapping Of The Plasmid Construct With NheI-HF
April 23, 2018
I have designed and constructed a donor plasmid of size approximately 19.4 Kb for cloning purposes. This plasmid prepped from E. ...
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Assay Kit/Reagent User Review
Diagnostic Restriction Mapping Of The Plasmid Construct PTS-Del With NdeI
April 20, 2018
I routinely construct series of several plasmids for cloning and expression purposes. In such cases, there is a necessity to check...
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Assay Kit/Reagent User Review
Diagnostic Restriction Mapping Of The Plasmid Constructs Using ZraI Restriction Enzyme
April 19, 2018
I have constructed a plasmid of size 6788 bp for cloning. This plasmid was transformed into E. coli DH5 alpha cells and selected ...
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Assay Kit/Reagent User Review
Removal Of Contaminating Nucleic Acids From Recombinant Protein Preparations
April 18, 2018
I have used Merckmillipore Benzonase nuclease to remove DNA and RNA from recombinant protein expressed in E. coli BL21 cells. ...
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Product Review
Efficient and Cost Friendly SYBR Green Mastermix for qPCR
Parijat Sarkar
I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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