Good Immunofluorescence Staining

Neuroscience
King's College London
PhD Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Purified anti-PSD95 Antibody [Clone: K28/43]

Catalog Number:

810401

I study genetics causes of neurodegenerative diseases. I am currently using primary rat cortical neurons to investigate dendritic spine morphology. I have used this antibody to stain for endogenous PSD9-95 as an indication of postsynaptic proteins. This antibody works very well and is recommended by my colleague.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Rat cortical neurons

Primary Incubation

1:500, overnight, 4C

Blocking Agent

5% BSA

Secondary Incubation

Alexa Fluor 568, 2 hours, room temperature (1:500)

Tertiary Incubation

N/A

Detection

Immunofluorescence

Results Summary

Good signal to noise intensity.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Nice staining

The Bad

No

The Bottom Line

Recommended

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