Invitrogen Antibodies<
HIF1A Monoclonal Antibody (mgc3)
MA1-516
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HIF1A protein plays a significant role during hypoxic conditions and can modulate the transcription of several genes involved in angiogenesis and metastasis. We used this antibody for probing HIF1A in MCF7 cell lysates after treatment with our molecule of interest.
Western Blot
Cell Lysates of MCF7
1:1000 dilution prepared in blocking solution, incubated overnight at 4 degrees
5% skimmed non-fat milk in PBST20
1:5000 anti-mouse HRP antibody, incubated for an hour at RT
None
ECL based detection was used
We observed the right size band (116 kDa) and upon treatment, we could see the reduction in HIF1A protein levels.
N/A
Good antibody
Bands observed were not crisp.
Overall, its a good antibody for Western Blotting.