CD3 PerCP-Cy5.5

January 24, 2018

Immunology
The Jackson Laboratory for Genomic Medicine
Lab Manager, RA III

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

PerCP-Cyanine5.5 Anti-Human CD3 (UCHT1)

Catalog Number:

65-0038-T100

CD3 PerCP Cy5.5 (clone UCHT1) is part of a phenotyping panel used on 500K PBMC freshly isolated from asthmatic patient's blood. In this panel we stained for T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

1/25 dilution in PBS+ 2%FBS for 15min at RT at dark.

Blocking Agent

FcR block (Miltenyi), 3min

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSR II

Results Summary

Good CD3+ T cells separation

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

N/A

The Bottom Line

Good and easy separation of CD3+ cells

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