CD11c to Stain for DCs

February 28, 2018

Immunology
The Jackson Laboratory for Genomic Medicine
Lab Manager, RA III

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-mouse CD11c Antibody

Catalog Number:

117307

This antibody was used to stain BMDC for CD11c+ cells and other surface molecules to make sure they differentiated correctly in culture during a survival assay.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse BMDC

Primary Incubation

1/50 dilution in PBS+ 2%FBS for 15min at RT at dark.

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII

Results Summary

Good separation of Dendritic Cells from macrophages in the culture

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good and bright antibody

The Bad

None

The Bottom Line

Good and bright antibody for BMDC separation

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