Antibody To Detect NK Cells

January 19, 2018

PRIMATE RESEARCH CENTER
UNIVERSITY OF WISCONSIN
Scientist I

Overall

Quality of Results

Ease-of-Optimization

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Company:

Beckman Coulter

Product Name:

CD159a antibody

Catalog Number:

A60797

We used this antibody to detect NK cells in peripheral blood from cynomolgus monkey. After ficoll separation of the MNCs, the percentage of NK cells in the MNCs was detected using the CD159a antibody. This clone of the antibody cross reacts with NHPs making it a suitable antibody for detecting NK cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMNCs from NHP

Primary Incubation

3ul of antibody for 100ul of cells in FACS buffer for 15 mins at RT

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

MAQSQuant

Results Summary

The antibody was very easy to use and gave very good separation of NK cells from the pool of lymphocyte population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very easy to use, good separation

The Bad

None

The Bottom Line

A very good antibody for the detection of NK cells.

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