BioLegend
PE/Cy7 anti-mouse Podoplanin Antibody
127411
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: Podoplanin Flow Cytometry Antibodies
In this experiment we were examining type II alveolar epithelial cells using podoplanin in WT and KO mice.
Flow Cytometry
Digested lung from mouse
1 ul per 100 ul reaction, incubated 30 minutes at 4 degrees in the dark
Fc Block
None
BD LSR II
The antibody clearly worked and showed a strong positive signal, but I was expecting more distinct populations as opposed to diffuse staining intensity. I was not satisfied with the results, although it is possible that the digestion method altered the podoplanin (T1alpha) expression.
N/A
It worked and signal intensity was good
Staining was diffuse instead of clear populations
This antibody was OK, it did not provide the clear results I was hoping for but it clearly worked.