R&D Systems
Mouse ST2/IL-33 R Alexa Fluor® 700-conjugated Antibody
FAB10041N-100
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Browse Similar Products: ST2 Flow Cytometry Antibodies
In this experiment we were looking at ST2 expression on bone marrow stem cells in WT and KO mice.
Flow Cytometry
Bone marrow from mouse
8 ul per 100 ul reaction, incubated 30 minutes at 4 degrees in the dark
Fc Block
None
BD LSR II
We used a very large amount of of antibody but did not find a convincing positive population. I recommend using a different clone and a brighter fluorophore.
N/A
None.
Used a very high concentration of antibody with weak results.
Use a different clone and brighter fluorophore.