FITC Hamster IgG1 kappa Isotype Control For Use With Flow Cytometry

December 27, 2017

BlueskyReddit
Pharmacology and Experimental Neuroscience
University of Nebraska Medical Center
Graduate Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC Hamster IgG1 kappa Isotype Control

Catalog Number:

553971

The FITC Hamster IgG1 κ Isotype Control is utilized in this particular study to evaluate the phenotype of CD4+ effector T cells after polarization to a respective phenotype. This particular control was used to help distinguish the background fluorescence from the specific antibody signal.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

C57BL/6 isolated CD4+ T cells

Primary Incubation

0.1ug/1.0x105 cells for 1 hour

Blocking Agent

Gamma globulin

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS Calibur

Results Summary

This antibody worked excellently to disinguish the amount of background signal to noise ratio in order to determine the specific antibody signal.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The isotype seems to work well with minimal optimization needed

The Bad

N/A

The Bottom Line

This is a great control to use to distinguish your background signal to noise ratio.

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