Excellent CD4 Staining On Human PBMCs

December 19, 2017

BlueskyReddit
Infection and Immunity
University College London
PhD Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-human CD4 Antibody

Catalog Number:

344605

The aim was to observe HIV infection in human CD4+ T cells. I selected this product as Biolegend offers high quality antibodies at good prices.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

1:200 (50 ul), 30min, 4deg

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD Fortessa-X20

Results Summary

We can observe very good staining of CD4 on T cells.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Very good staining at high dilution. Very cheap

The Bad

None

The Bottom Line

I would recommend this product for flow cytometry.

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