Good CD103 Staining On Human PBMCs

November 30, 2017

Infection and Immunity
University College London
PhD Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 605 anti-human CD103 (Integrin αE) Antibody

Catalog Number:

350217

The aim of this study was to observe expression of CD103 on human CD4+ T cells when cultured in vitro. I selected this product as Biolegend offers high quality antibodies at good prices.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

1:50 (50ul), 30min, 4deg

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD Fortessa-X20

Results Summary

We cannot observe CD103 expression on CD4+ T cells; however, we can see good expression on the CD4- population (most likely CD8+ T cells).

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Bright staining

The Bad

None

The Bottom Line

I would recommend this product antibody for flow cytometry.

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