Anti-Mouse FOXP3 Antibody

November 10, 2017

BlueskyReddit
Harvard Medical School
Harvard
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-mouse FOXP3 Antibody

Catalog Number:

126404

I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine single cells suspension

Primary Incubation

1:100 dilution for 1 hour on ice

Blocking Agent

Blocking with 2% BSA and Fc block (1:100)

Secondary Incubation

1:50 dilution for 1 hour on ice

Tertiary Incubation

None

Detection

FACS

Results Summary

Nuclear FOXP3 signal was detected in samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good antibody for efficient detection of FOXP3 on mice cells.

The Bad

None

The Bottom Line

Good nuclear staining for FOXP3 to detect T regulatory cells.

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