Excellent Antibody For Human CD134 Staining

October 17, 2017

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Invitrogen Antibodies

Product Name:

CD134 (OX40) Monoclonal Antibody (ACT35 (ACT-35)), PE

Catalog Number:

12-1347-42

I used this antibody to characterize OX40 expression in human thymocytes under steady state. This antibody worked well and gave a nice distinct population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human thymocytes

Primary Incubation

1:100 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This antibody stained well and gave a clear distinct population. I was able to generate presentable figures without much optimization hurdles.

DOI or PMID #

NA

Additional Notes

Surface staining of CD134 before Fixation gives better staining

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Expensive

The Bottom Line

Good quality antibody and worth buying it

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