Excellent Flow Cytometry Antibody For Human EpCAM

September 25, 2017

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Biolegend

Product Name:

Pacific Blue™ anti-human CD326 (EpCAM) Antibody

Catalog Number:

324217

I used this antibody to characterize human thymic epithelial cells. We defined thymic epithelial cells as CD45-EpCAM positive. This antibody stained well and gave us nice distinct population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human thymus

Primary Incubation

1:100 for 1h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This antibody stained well and gave a clear distinct population. I was able to generate presentable figures without much optimization hurdles.

DOI or PMID #

NA

Additional Notes

Thymic epithelial cell preparation requires liberase digestion

Related Categories

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Summary

The Good

Ease of optimization and cheap

The Bad

NA

The Bottom Line

Good quality antibody and worth buying it.

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