Biolegend
CD69 antibody
310909
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Browse Similar Products: CD69 Flow Cytometry Antibodies
Cynomolgus peripheral blood was used to isolate mononuclear cell cultures (MNCs) using Ficoll gradient centrifugation. The MNCs were then activated using Con A for 3 days and the activation of cells was checked using CD69 antibodies.
Flow Cytometry
Peripheral Blood Mononuclear Cell Cultures from Non-Human Primates
5ul of antibody for 100ul of cells in FACS buffer for 15 mins at RT
None
MAQSQuant
There was a clear separation between the positive and negative population.
N/A
Very easy to use and optimize
The antibody is very good for detecting activated T cells.