Invitrogen Antibodies by Thermo Fisher Scientific
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488
A32723
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This secondary antibody was used to perform indirect immunofluorescence microscopy on HeLa cells to detect the ER/mitochondria. It showed strong immunoreactivity that was consistent mitochondria. Background levels were low. This antibody is a good choice for immunofluorescent microscopy.
Immunofluorescence
HeLa cells fixed with 3.7% paraformaldehyde
1 hour at room temperature in blocking agent at 1:300
PBS in 1% normal goat serum and 0.1% triton x-100
1 hour at room temperature (1:300)
DAPI
Confocal microscopy
This secondary was specific and bright in HeLa cells. It is a good choice but it is more expensive than A27034.
N/A
Works well
Premium cost
This secondary works well, but it is more expensive than its non plus version. The results are comparable between the two