Qiagen
RBP-Jk Reporter
CCS-014L
Notch transcription can be easily detected with the RBP-Jk reporter tagged by luciferase. It is a great reporter for detecting effects on transcription by drug treatment as well.
Reporter Assays
Human breast cancer cells
Transfect cancer cells with reporter by lipofectamine for 24-48 hrs prior to dectecting on luminometer
Optimization of cell densities must be performed to ensure best transfection efficiency
Rel luc activity is firefly divided by renilla luciferase counts. This can be used to compare Notch transcription levels. Higher levels were seen compared to negative controls in a couple cell lines.
N/A
Easy to use
None
Great assay for detecting Notch transcription with minimal trouble.