Biolegend
APC anti-mouse CD185 (CXCR5)
145505
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The goal of the project was to measure Tfh cells after immunizing mice.
Flow Cytometry
Mouse spleen
1: 100, 4 degrees, 30 min
Fc block
None
Good staining allows for clear demarcation of PD-1hiCXCR5hi Tfh cells.
N/A
Easy to use, distinctly marks positive cells
Good antibody for Tfh cell staining.