Biolegend
Brilliant Violet 421™ anti-human CD69
310930
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Browse Similar Products: CD69 Flow Cytometry Antibodies
One of our lab focuses is the isolation and characterization of different effector T cell subsets in various human tissues. We need good antibodies to identify the different subsets to allow for downstream analysis and comparison. This antibody is very bright and allows us to see CD69+ T cells in lung cells even when the numbers are low, without too much background or spillover.
Flow Cytometry
Human lung lymphocytes
Cells were stained in 100uL FACS buffer with antibody at 1:200; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS buffer
None
LSR II
Using this antibody we could successfully identify the CD69+ from the negative populations in our human lung cell samples.
N/A
Very bright
Low spillover
This antibody works well and is bright enough to see small populations.