Human NPTX1 ELISA Kit (Sandwich ELISA) Gives Reliable Data

Protocol Overview

1O0 μL of supernatant sample was added to anti-NPTX1 antibody-coated plate and incubated for 1 hour at 37°C. All liquid was then removed and the plate manually washed 5 times with wash buffer. 100 μL of enzyme conjugate was then added and incubated for 1 hour at 37°C. After washing again, color solution was added at 100 μL per well and incubated for 30 minutes at room temperature. Following an additional five washes, the plate was incubated with 90 μL of TMB substrate for another 15 minutes at 37°C in the dark. The reaction was then stopped by addition 100 μL of stop solution. Absorbance was measured at 450nm using a SpectraMAX Plate Reader. Data was analyzed using SoftMax Pro software.