Rabbit Anti-HA Antibody From Cell Signaling Technology

April 19, 2017

Biology
Franklin and Marshall College
assistant professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

HA-Tag (C29F4) Rabbit

Catalog Number:

3724

This antibody (3724) was used to perform immunofluorescence microscopy on HeLa cells transfected with an HA-tagged construct and an endoplasmic reticulum resident protein. 3724 showed moderate to strong immunoreactivity. Background levels were low. This antibody is a good choice for multicolor microscopy.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Hela cells fixed with 3.7% paraformaldehyde

Primary Incubation

1 hour at room temperature in blocking agent at 1:300

Blocking Agent

PBS +1% normal goat serum and 0.1% triton x-100

Secondary Incubation

Goat anti-rabbit conjugated to Alexa 647

Tertiary Incubation

DAPI

Detection

Confocal microscopy

Results Summary

The rabbit anti-HA from Cell Signaling is a good option for a rabbit antibody to HA. It has strong immunoreactivity for overexpressed constructs.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong and specific staining

The Bad

Expensive

The Bottom Line

Although this antibody is expensive, it works well if you are looking for a non-mouse anti-HA antibody

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