Good Anti-CD45.2 Antibody For Flow Cytometry

Immunology
UT MD Anderson Cancer Center
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Tonbo Biosciences

Product Name:

APC Anti-Mouse CD45.2 (104)

Catalog Number:

20-0454

We created a bone marrow chimeric mice by adoptive transfer of CD45.2+ bone marrow cells into lethally irradiated CD45.1+ recipients. Six weeks later, peripheral blood was analyzed for CD45.2+ reconstitution by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse peripheral blood cells

Primary Incubation

APC anti-mouse CD45.2, 20 min @ 4C

Blocking Agent

FcR blocking antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry LSRII

Results Summary

This antibody clearly distinguish donor-derived CD45.2+ population (>90%) from CD45.2- host cells in peripheral blood of bone marrow chimeric mice.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Strong signal, and clear separation

The Bad

N/A

The Bottom Line

N/A

Share your experience with other scientists. Write a Review! »

Join the discussion