Miltenyi Biotec
CD16 MicroBeads, human
130-045-701
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Our laboratory investigates different ways to improve immune responses against HIV, including enhancement of NK mediated ADCC activity. We use these beads to deplete cells expressing Fc gamma receptor (mostly NK cells) and therefore eliminate ADCC activity as a control in our assays.
Cell purification
Human PBMCs
Resuspend the cells in 50ul of buffer per 5*10^7 cells. Add 50ul of beads per 5*10^7 cells. Incubate at 4C for 30min. Wash cells, resuspend at 1*10^8/500ul. Apply to column on magnet.
Follow the protocol.
Good depletion of CD16+ cells
N/A
Consistent, easy to use
Expensive
Great product to use for depletion of isolation of CD16 expressing cells