Vector Laboratories
VECTASTAIN ABC HRP Kit (Peroxidase, Mouse IgG )
PK-4002
Info: View Product View Product Specs
Our lab routinely performs IHC on mouse CNS tissue to assess axonal damage and neuroinflammation. To visualize staining, we use the Vector HRP kits and develop sections with DAB. This kit delivers quality results and is easy and simple to use.
Immunohistochemistry
Frozen and paraffin-embedded coronal mouse brain sections
1) Incubate sections in primary antibody then wash 2a) Incubate sections in anti-mouse IgG biotinylated secondary antibody solution2b) While sections incubate in secondary solution, mix A+B solution, incubate at room temperature for 1hr3) Wash sections following secondary antibody incubation then incubate sections in A+B solution for 30min at room temperature4) Wash sections then incubate in DAB5) Coverslip in Permount
Optimize timing in DAB solution so that sections are not overstained
Staining will appear brown and can be visualized via light microscopy
N/A
Provides great staining of sections
A lot of steps
Great for anyone wanting a reliable kit to stain and visualize sections using DAB
Product Name: VECTASTAIN® ABC-Peroxidase Staining Kit (Mouse IgG)Supplier Page