Great ELISA Antibody

March 16, 2017

Microbiology
Medical College of Wisconsin
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Jackson Immunoresearch

Product Name:

AffiniPure Goat Anti-Mouse IgG, Light Chain Specific

Catalog Number:

115-005-174

Info: View Product View Product Specs

Browse Similar Products: IgG ELISA Antibodies

We study virus-host interactions using a mouse model of infection. Here we are examining total IgG concentrations in the serum of mice following infection with a chronic virus.

Experimental Design and Results Summary

Applications

ELISA

Sample

Mouse serum

Primary Incubation

10ug/mL - 4C overnight

Blocking Agent

PBS with BSA(3%) and tween(0.05%)

Secondary Incubation

100uL mouse serum diluted 1:30,000, or standards - RT for 1-2 hours

Tertiary Incubation

100uL detection antibody diluted 1:5000 - 1hour at RT

Detection

TMB substrate

Results Summary

Sensitive antibody for use in ELISAs. We are able to detect significant changes in serum IgG concentrations.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Great antibody

The Bad

N/A

The Bottom Line

Works great if used as recommended.

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