Amylase Activity Assay

February 28, 2017

Children's Hospital Los Angeles
Urology
Research Specialist II

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma Aldrich

Product Name:

Amylase Activity Assay Kit

Catalog Number:

MAK009

The aim of this research study is to establish a model to study mechanism and prevention for chronic pancreatitis. This product was used to determine the damage caused by drug to verify the amylase levels in serum plasma in rodents. This product is accurate; the protocol is simple, great results and the experiment is finished in a short amount of time.

Experimental Design and Results Summary

Application

Assay

Starting Material

Mouse serum plasma

Protocol Overview

We followed the instructions from the kit. For our experiment we used serum plasma for the assay.

Tips

Start thawing the Amylase Buffer right away, it takes a long time to thaw

Results Summary

The assay is very quick and easy to follow. The calculations are very simple, but it does help to have a template on excel if your repeating the experiment several times.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

The experiment takes less than 20 minutes to read, it does depend on the amount of sample used.

The Bad

The limiting reagent is Amylase Substrate Mix, I would highly recommend running the whole plate at a time.

The Bottom Line

The assay works very well. It is simple, and the protocol is very easy to follow.

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