Staining Mouse Splenocytes With Anti-H2Kb FITC

March 31, 2017

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

anti-mouse H2Kb FITC

Catalog Number:

116505

This antibody was used as a donor cell marker in mouse splenocytes for allogeneic transplant studies. It was chosen because it is a commonly used clone for H2Kb, FITC works well with other fluorophores in the staining panel, and Biolegend had a great price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 dilution in 100uL total staining volume for 15 minutes at room temperature.

Blocking Agent

FcBlock + 2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

N/A

Results Summary

H2Kb staining was great! Positive cells were fully resolved from negative.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well, low cost.

The Bad

N/A

The Bottom Line

Will continue to use this antibody.

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