Tonbo Biosciences
FITC Anti-Human CD45RO (UCHL1)
35-0457-T025
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FITC Anti-Human CD45RO (UCHL1) was used in a sorting panel to separate memory and naive T cells.
Flow Cytometry
Human PBMC from whole blood
PBMC were stained with an antibody cocktail containing CD45RO FITC (clone UCHL1) dilution 4/100 for 15min at RT at dark
FcR block (Miltenyi), 3min
N/A
LSRII
Easy separation of memory and naive T cells in order to sort naive T cells
Nice separation
The separation looks normal and the memory and naive T cells are well separated and easy to gate.