VEGFR2 Antibody For Microsopy

December 20, 2016

Physiology and Biophysics
Albert Einstein College of Medicine
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Human VEGF R2/KDR/Flk-1 Antibody

Catalog Number:

AF357

VEGF2 antibody to detect microtubule pool of VEGFR2 in HUVECs

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PFA fixed HUVECs

Primary Incubation

Overnight, 1:200

Blocking Agent

BSA

Secondary Incubation

2 hours, 1:500

Tertiary Incubation

NA

Detection

Perkin Elmer spinning disc confocal microscope

Results Summary

The microtubule pool of VEGFR2 is detected using this antibody. colocalisation can be quantified by co-staining for tubulin.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Easy to optimize

The Bad

Signal can be low

The Bottom Line

Best antibody I've come across for VEGFR2 imaging

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