Bioline
SensiFAST SYBR Hi-ROX Kit
BIO-92020
Our lab switched to this since it was cheaper than other SYBR qPCR master mixes and works just as well.
qPCR
cDNA
Use 1ul of cDNA, 3 ul of 2X master mix, .4 uM primers, and H2O to 6ul.
None
Gives good consistent signal
N/A
Cheaper than other mixes
If you are looking for cheap qPCR SYBR mastermix I would recommend this one from bioline.