Great Actin Antibody

December 14, 2016

BlueskyReddit
Urology
UCSF
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-beta Actin antibody

Catalog Number:

ab8226

I use this antibody against mouse beta actin for most of my loading controls for western blots. It gives good signal with no background.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse embryonic stem cell

Primary Incubation

1:2000, overnight at 4 degree

Blocking Agent

Odyssey blocking buffer

Secondary Incubation

1:10,000 2 hours at room temp

Tertiary Incubation

None

Detection

Licor

Results Summary

Good signal with no background

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well. Good clean signal

The Bad

A bit expensive

The Bottom Line

I would recommend for beta actin western blots

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