Highly Sensitive ECL Solution

Texas Tech University Health Science Center
Internal Medicine
Postdoctoral Researcher

Overall

Quality of Results

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Company:

Thermo Scientific

Product Name:

Super Signal West Dura Extended Duration Substrate

Catalog Number:

34075

Proteins which are low in occurence give out weak signals which cannot be detected properly. This high sensitive ECL is highly sensitive and hence used to detect the signal for proteins.

Experimental Design and Results Summary

Application

Western Blotting

Starting Material

Mouse cell lysate

Protocol Overview

High sensitive ECL is easy to use and detects the signal efficiently. Use 2ml of supersignal west dura stable peroxide buffer and 2ml of super signal west dura enhancer solution and keep it shaking slowly for 2min and detect the band using X-ray film.

Tips

Use low amount as much as possible because the signal will be very strong sometimes we will get background.

Results Summary

Very prominent and effective.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Very good for western blot

The Bad

None

The Bottom Line

Great product. Highly recommended.

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