Excellent Application In IHC

HMG
VCU
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-smooth muscle actin antibody

Catalog Number:

ab5694

My research focus is to evaluation the development of cancer cell in animal tissue. Doing IHC for SMA could help me to define the development stage of cancer. Compare with other companies antibody, I thought Abcam was the company I trust and the outcome turns out that I am not wrong.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse prostate tissue

Primary Incubation

1:5000, 4℃ overnight

Blocking Agent

The same source with secondary antibody

Secondary Incubation

Ready to use, room temperature 30 min

Tertiary Incubation

Stain for 10 seconds to 15 seconds

Detection

DAB

Results Summary

The results were excellent. Clear background, strong stain signal of target protein.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The volume is big, about 500 micro liters, the dilution is 1:5000, this antibody is enough for plenty application of IHC.

The Bad

Not found yet

The Bottom Line

Easy to use and with high quality stain results.

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