IL-7R BV421 for ILCs

Immunology
The Jackson Laboratory for Genomic Medicine
Lab Manager, RA III

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BV421 Mouse Anti-Human CD127

Catalog Number:

562437

This antibody was used to sort ILC2 from whole blood in order to expand them and to send them for RNA seq in order to create an Immune blood bank for RNA seq. This antibody works really well to separate ILC2 from other ILCs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs from whole blood

Primary Incubation

2.4/100 dilution in PBS+ 2%FBS for 15min at RT at dark.

Blocking Agent

FcR Block (Miltenyi) for 3min at RT

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Aria fusion

Results Summary

ILC2 were nicely separated and sorted gating on CRTH2 as well

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Bright antibody

The Bad

None

The Bottom Line

CD127 BV421 works well for human PBMC

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