Excellent CD3 antibody

Pulmonary
Harvard Medical School
Post Doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD

Product Name:

FITC Mouse Anti-Human CD3

Catalog Number:

561806

We used this antibody for immune phenotypic characterization of peripheral blood of children with arthritis. We needed to use reliable antibodies because we were limited to small volumes of blood.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human Peripheral blood

Primary Incubation

The antibody was incubated directly on peripheral blood for 30 minutes. After that we performed red blood lysis using a BD lysis buffer. Cells were washed twice with PBS and then taken directly to the flow cytometer

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

This is an excellent antibody to identify CD3+ cells..

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Reliable staining of CD3+ peripheral blood cells

The Bad

Nothing

The Bottom Line

This is an excellent antibody to identify CD3+ cells

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