Excellent Anti-Mouse CD8 Antibody

Biomedical Sciences
TTUHSC
Lead Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PE Rat Anti-Mouse CD8a

Catalog Number:

553033

Balb/C mice were sacrificed and single cell suspensions prepared from the spleens were used for flow cytometric analysis. This antibody was used for detecting mouse CD8 expression in combination with anti-mouse CD4 antibody. The results showed clear CD4 and CD8 positive populations which confirms the specificity of this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

30

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry in PE Channel

Results Summary

Clear CD8 positive population confirms the specificity of this antibody

Additional Notes

None

Related Categories

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Summary

The Good

Great specificity

The Bad

None

The Bottom Line

Good anti-mouse CD8 PE antibody

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