Staining AML Cell Line With Anti-CD33 PE

August 17, 2016

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-human CD33 PE

Catalog Number:

303404

This antibody was used in combination with anti-human CD45 to as a marker of AML in a xenograft mouse model. It was chosen because it is a commonly used clone of CD33, PE is a bright fluorochrome, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from AML model mouse

Primary Incubation

5uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

1:50 dilution of anti-mouse and anti-human FcBlock and 2% FBS.

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

CD33 staining was excellent, as nearly all AML cells recovered from the spleen were positive for CD33.

Additional Notes

None

Related Categories

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Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

I will continue to use this antibody.

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