Staining PBMC With Anti-CD56 PE/Cy7

August 08, 2016

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-human CD56 from Biolegend

Catalog Number:

318317

This antibody was used as a marker for NK cells in combination with CD3 to distinguish NKT cells from T cells in human PBMC. It was chosen because it is a commonly used clone for CD56, PE/Cy7 is a great "dump channel" in flow cytometry, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

5uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

CD56 staining was as expected, and NK cells, NKT cells and T cell populations were easily resolved from each other.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

I will definitely keep using this antibody.

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