Biolegend
Anti-mouse TER-119 FITC
116206
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This antibody was used as a RBC marker in a hematopoietic lineage panel. It was chosen because it is a commonly used clone, spillover from FITC staining to other markers will be minimal since TER-119 is specific to RBC, and Biolegend had the best price.
Flow Cytometry
peripheral mouse blood
1uL per test in 100uL total staining volume for 20 minutes at room temperature
1:50 dilution of anti-mouse FcBlock and 2% FBS.
n/a
TER-119 staining was as expected, with 100% of RBC exhibiting a strong signal in the FITC channel compared to isotype control.
None
Works well. Low cost
I will continue to use this antibody in this panel.