Fig 1: Infiltrating Monocytes Contribute to Three Subsets of Macrophages during Myocarditis(A) Schematics of parabiosis mice.(B) Representative images of H&E-stained heart sections of the median CD45.1 (day 21 EAM) and CD45.2 (non-EAM) mice parabionts. Scale bars: 100 µm.(C) Comparison of total grafted CD11b+Ly6G-myeloid cell counts between parabionts.(D) Comparison of total cardiac macrophage counts between parabionts.(E) Flow cytometry plots showing (top left) percentage of CD45.2+CD11b+Ly6G-Lineage (CD3e, B220, NKp46, CD90.2, and Ter119)- grafted cells infiltrating the CD45.1 EAM hearts; (top middle) percentage of grafted cells in the hearts that differentiated into macrophages or remained as monocytes; (top right) percentage of grafted MDM subsets; (bottom left) percentage of CD45.1+CD11b+Ly6G-Lineage grafted cells infiltrating the CD45.2 non-EAM hearts; (bottom middle) percentage of grafted cells in the hearts that differentiated into macrophages or remained as monocytes; and (bottom right) percentage of grafted MDM subsets.(F) Percentages of grafted MDMs out of total number of grafted CD11b+Ly6G-Lineage- myeloid cells.(G) Percentages of grafted Ly6Chi and Ly6Clo cells out of total number of grafted F4/80-CD64+ monocytes.(H) Comparison of grafted MDM subsets defined by CCR2 and MHCII expressions between parabionts. Data are representative of two independent experiments with biological triplicates. n = 3.(C, D, F, G) Groups were compared using Student’s t test. *p < 0.05. All data were presented as mean ± SD.See also Figure S1.