Staining Mouse WBC With Anti-CD4 PerCP/Cy5.5

August 01, 2016

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

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Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-mouse CD4 PerCP/Cy5.5

Catalog Number:

100539

This antibody was used to phenotype CD4+ T cells in peripheral mouse blood. It was chosen because it is a commonly used clone, PerCP/Cy5.5 is bright, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

ACK-lysed peripheral mouse blood

Primary Incubation

1uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

1:50 dilution of anti-mouse FcBlock and 2% FBS.

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

CD4 staining was as expected.

Additional Notes

None

Related Categories

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Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

I will definitely use this antibody again.

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