Bioline
Tetro cDNA Synthesis kit
BIO-65042
Bioline's Tetro cDNA synthesis kit was used to synthesis first strand cDNA using Salmonella enterica total RNA to quantify gene expression. A total of 1 microgram RNA was used and at the end of the reaction, I got an yield of 4 microgram of cDNA in the 20 microliter reaction volume. Gene expression experiments with cDNA went well in qPCR.
cDNA synthesis for qPCR
Salmonella enterica total RNA
A total of one microgram RNA from Salmonella enterica total RNA samples were used for cDNA synthesis using random hexamer primers to prime the cDNA synthesis in a 20 microliter reaction volume. Synthesis steps, temperature cycles are followed according to the kit's instructions. At the end of the reaction, quality and quantify of cDNA was checked by Nanodrop spectrophotometer.
If you have multiple total RNA samples with different quantities, then normalize input quantities to be used across samples. Try to use same amount of input RNA in the reaction.
One microgram total RNA input was used in the cDNA synthesis reaction and yield was very good with 4 microgram. This was sufficient for my all qPCR experiments. I used 1:10 diluted cDNA as template in the qPCR assays.
None
Protocol is very simple and easy to perform. Not that much expensive.
This kit performs well with respective synthesis of good quality cDNA for qPCR experiments.