Dead Cell Separation from Single Cell Preparation

The Jackson Laboratory
Cancer biology
Post doc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Miltenyi Biotec

Product Name:

Dead Cell Removal Kit

Catalog Number:

130-090-101

We prepare single cells from various tissues for our genomic studies. We encountered lot of damaged and dead cells in the end from the preparation. This kit works on MACS principle, fairly simple and easy to use. We use this kit to remove dead cells from the cells suspension as part of our procedure.

Experimental Design and Results Summary

Application

Dead cell removal

Starting Material

Cells suspension

Protocol Overview

The Cells suspension (1-10million cells) lung/brain, blood was mixed with binding buffer and incubated for 20 min at RT. The Cell-beads suspension was pass through MS columns, effluent collected for viable cells.

Tips

Optimum condition - Room temperature

Results Summary

We obtained final suspension that shows 70-80% reduction of dead cells from the initial suspension.

Additional Notes

None

Image Gallery

Summary

The Good

Easy, less time consuming

The Bad

Expensive, optimal performance

The Bottom Line

The kit works well in short span of time and very easy to use.

Join the discussion