Dead Cell Removal Kit from Miltenyi Biotec

(1) Protocol for culture, drug treatment, and dissociation of patient-derived 3D ovarian cancer microspheroids for flow cytometrySTAR ProtocolsJune 1, 2026Mariam Haffa, Karolina Juhani, Greta Gudoityte, Rita Hutyra-Gram Ötvös, Emelie Wallin, Olli-Pekka Kallioniemi, Josefin Fernebro, Ulrika Joneborg, Brinton Seashore-Ludlowtubes,Merck,Cat#CLS3213 TrypLE™ Express Enzyme 1X,Thermo Fisher Scientific,Cat#11538856 Dead Cell Removal Kit,Miltenyi,Cat#130-090-101 LS columns,Miltenyi,Cat#130-042-401 QuadroMACS™ Separator,Miltenyi,Cat#130-090-976 96-well V-shape

(2) Enhanced endocrine–metabolic support and axonemal assembly in high-sperm-motility geese: insights from testicular cellular heterogeneity by scRNA-seqPoultry ScienceJune 1, 2026Hechuan Wang, Kexin Cong, Jiaxin Yin, Ke Jiang, Yunuo Liu, Xiaofang Ren, Ying Zhang, Shengjun LiuRhine-Westphalia, Germany) and centrifuged at 4°C (300 × g, 5 min). Dead cells were removed using a Dead Cell Removal Kit (Miltenyi Biotec, North Rhine-Westphalia, Germany) and passed through MS columns (Miltenyi Biotec, North

(3) Single-cell RNA-sequencing of dermal fibroblasts demonstrates culture-induced changes and variable persistence of keloid disease featuresiScienceMay 15, 2026Amy Lock, Elena M. Drudi, Dasha Freydina, Brian M. Stramer, Franziska Denk, Tanya J. Shawvials were thawed and enriched for live cells using the MACS Dead Cell Removal Kit (Miltenyi Biotec, #130-090-101). To study the effects of matrix deposition on fibroblast phenotype, a protocol based on cell-derived

(4) Single cell transcriptional evolution of myeloid leukemia of Down syndromeNat CommunApril 23, 2026Mi K. Trinh, Konstantin Schuschel, Hasan Issa, Rebecca Thomas, Conor Parks, Agnes Oszlanczi, Toochi Ogbonnah, Di Zhou, Lira Mamanova, Elena Prigmore, et al.through a 70μm cell strainer (Falcon) and washed with PBS. If necessary, live cell enrichment using a Dead Cell Removal kit (Miltenyi Biotec) and red blood cell removal using the eBioscience 10X RBC Lysis Buffer (Multi-species)

(5) Immunogenic tumor cell death and T-cell-derived IFN-γ elicit tumoricidal macrophages to potentiate OX40 immunotherapyCell Reports MedicineApril 21, 2026Yanqin Liu, Jiliang Zhao, Kailu Yang, Qiongqiong Ma, Dongping Zhang, Lili Xu, Zhaoyuan Zhang, Zhongqian Yin, Jingru Chen, Yi Wang, et al.221000 Mouse CD45(TIL)MicroBeads,Miltenyi Biotec,Cat# 130-110-618 Dead Cell Removal Kit,Miltenyi Biotec,Cat# 130-090-101 Chromium Single Cell 5′ Reagent Kits User Guide (v2 Chemistry Dual Index),10x Genomic,Cat# PN-1000263 SPARKeasy

(6) Single-Cell Transcriptomic Profiling of Ectopic ACTH-Secreting Pheochromocytoma Reveals the Chromaffin Cell Origin of Ectopic Hormone ProductionInt J Mol SciApril 18, 2026Xu Wang, Penghu Lian, Guoyang Zheng, Wenda Wang, Yang Zhao, Yushi Zhangthe cell pellet was collected after centrifugation. Add 100 μL of Dead Cell Removal MicroBeads (MACS 130-090-101; Miltenyi Biotec, Bergisch Gladbach, Germany), mix well, and incubate for 15 min at room temperature.

(7) A multimodal atlas for immunotherapeutic targeting of AML surface heterogeneityiScienceApril 17, 2026Matthew Ung, Julia Etchin, Amanda Halfond, Julia DiFazio, Yonina Keschner, Alyssa Pyclik, Anne Campbell, Ruijia Wang, Mariana Silva, Brikena Gjeci, et al.RPMI 1640 (1X) (Gibco™) + 10% fetal bovine serum (FBS) (Corning). Live BMMCs were enriched using the Dead Cell Removal Kit (Miltenyi Biotec) following the manufacturer’s protocol, passed through a magnetic column, and eluted

(8) Tumor reactivity assessment using clonal expression reveals tumor reactive CD8+ T cell heterogeneity across solid tumorsFront ImmunolApril 16, 2026David Monteiro, Jack Denebeim, Anne E. Dodson, Ashish Yeri, Mitali Ghose, Meghan Travers, Sophie Capobianco, Conor Calnan, Gustavo J. Martinez, Charles H. Yoon, et al.T cells from tumor starting material and from autologous co-cultures underwent dead cell removal (#130-090-101, Miltenyi Biotec) followed by T cell enrichment using CD3 microbeads (EasySep Human CD3 #17851, STEMCELL Technologies

(9) Histotripsy-Initiated Immune Response Synergizes with Chemotherapy in a Neuroblastoma Murine ModelCancers (Basel)April 15, 2026Natalia Antonides-Jensen, Muskan Singh, Yuqing Xue, Fernando Flores-Guzman, Lydia L. Wu, Samantha S. Yee, Jacky Gomez-Villa, Timothy L. Hall, Mark A. Applebaum, Kenneth B. Bader, et al.before proceeding with Dead Cell Removal Kit (Miltenyi Biotec, Bergisch Gladbach, Germany; cat. no. 130-090-101) per manufacturer’s instructions. Cell preparations with under 70% cell viability were excluded from

(10) Amelioration of acute liver failure by a cinnamic acid derivative through inhibition of the ROS-NETosis axisMol BiomedApril 13, 2026Jie Yin, Longjie Ding, Ziming Zhao, Xia Chen, Jianzheng Huang, Yang Xiao, Lianghu Gu, Xiaotian Zhang, Qingyi Tong, Yonghui Zhangresuspended in complete culture medium and depleted of dead cells using the Dead Cell Removal Kit (Miltenyi, 130–090–101). scRNA-seq analysis Library preparation and sequencing: Single-cell suspensions were barcoded using

(11) Genome to single-cell characterization of the MAPK family in bighead carp (Hypophthalmichthys nobilis) spleen during Aeromonas hydrophila challengeFront ImmunolApril 13, 2026Yang Mao, Xue Yu, Wenjing Wang, Jin Peng, Minao Wang, Jianyun Zhou, Defeng LiBSA) (50). Viability was assessed by trypan blue (target ≥85%); a dead-cell removal kit (Miltenyi, 130-090-101) was used only if required. Cell concentration was adjusted to 700–1,200 cells/µL. 2.9 Single-cell

(12) Protocol for generating and characterizing Matrigel-free prostate cancer patient-derived organoidsSTAR ProtocApril 7, 2026Robin Dolgos, Romuald Parmentier, Jing Wang, Arnoud J. Templeton, Kirsten D. Mertz, Heike Pueschel, Helge Seifert, Ashkan Mortezavi, Tatjana Vlajnic, Cyrill A. Rentsch, et al.DMEM/F12,Gibco,12634010 Red Blood Cell Lysis Buffer,Roche,11814389001 Dead Cell Removal Kit,Miltenyi Biotech,130090101 Primocin,InvivoGen,ant-pm-1 Penicillin-Streptomycin,Sigma-Aldrich,P4333-100ML Collagenase Type 4,Worthington,LS004189 DISPASE,Gibco,17105041 Normal Goat Serum

(13) Genomic characterization of rabies virus glycoprotein co-expressing CD70 CAR-T cells during killing of glioma cells in vitroFront ImmunolApril 6, 2026Feng Ji, Kexing Gao, Xianchen Wu, Hao Lin500 × g for 4 minutes. Prior to RNA extraction and sequencing, dead CAR-T cells were removed using a Dead Cell Removal Kit (Miltenyi Biotec, USA). The RNA products from the three replicates of CAR-T cells were sent for RNA sequencing

(14) Glandular Cells of Forest Musk Deer Autonomously Synthesize Sex Steroid HormonesBiology (Basel)April 6, 2026Xian An, Xiangyu Han, Jinming Huang, Zexiu Zhang, Zhiyi Lou, Jingyao Hu, Rongzeng Tan, Pengcheng Yang, Xinyue Dou, Habib Bati, et al.Shanghai, China) with acridine orange/propidium iodide (AO/PI) staining. Dead cells were removed using a Dead Cell Removal Kit (Miltenyi Biotec), and viable cells were resuspended in PBS + 0.04% BSA at a concentration of 700–1200

(15) Targeting TPO/MPL Signaling to Mitigate JAK2V617F-driven Cardiac Microvascular DiseasebioRxivApril 3, 2026Xiaoxi Yang, Kyla Masarik, Xiaochuan Sun, Fangfang Zhang, Kirsten Zheng, Haoyi Zheng, Huichun Zhanwashing, cell pellets were resuspended in 100ul of Dead Cell Removal Microbeads per 107 total cells (Cat. 130-090-101, Miltenyi Biotec) and incubated 15 minutes at room temperature. The mixture was diluted in 1x binding

(16) A single-cell and spatial transcriptomic atlas of human tuberculous constrictive pericardiumeBioMedicineApril 1, 2026Feng Xiong, Yangran Qi, Shuzhen Wang, Lijuan Zhang, Fuyi Cheng, Kunyue Tan, Jian Chen, Yali Lei, Wenjie Yang, Zelei Zhao, et al.erythrocytes (Solarbio R1010) and then dead cells removal was decided to be performed or not (Miltenyi 130-090-101). Finally, fresh cells were washed twice in the RPMI1640 (Gibco 11875119) and then approximately 1 ×

(17) Single-Cell Triomics Analysis of Tumor Cells Infiltrating Patient-Derived Breast Cancer ScaffoldsThe American Journal of PathologyApril 1, 2026Stefan Filges, Emma Jonasson, Maria Del Carmen Leiva Del Carmen Leiva Arrabal, Lisa Andersson, Anna Gustafsson, Dalia Dhingra, Pedro Mendez, Aik Ooi, Adam Sciambi, Göran Landberg, et al.(VWR/Avantor, Radnor, PA) and a 40 μm cell strainer (VWR). Dead cell removal was performed by using the MACS Dead Cell Removal Kit (Miltenyi Biotec, Bergisch Gladbach, Germany), according to the manufacturer’s instructions. Triomics

(18) Comparative single-cell atlases reveal injury-driven tubal epithelial regeneration as a window for ovarian carcinoma initiationbioRxivMarch 30, 2026Coulter Q. Ralston, Andrea Flesken-Nikitin, Dah-Jiun Fu, Christopher S. Ashe, Blaine A. Harlan, Md Mozammal Hossain, Derek K. Wang, Anna Yemelyanova, Elisa Schmoeckel, Andrew K. Godwin, et al.7 minutes at 4°C and subjected to a dead cell removal assay (Dead cell removal Kit, Miltenyi Biotec 130–090-101) following the manufacturer protocol. In short, cells were suspended in 100 μl microbeads, MS columns

(19) Acute Inflammation Drives Corneal Pathological RegenerationInvest Ophthalmol Vis SciMarch 30, 2026Zhuo Han, Huizhen Guo, Bofeng Wang, Yongxu Zhu, Xin Zhao, Jialin Chen, Kunlun Mo, Jieying Tan, Jianping Ji, Hong Ouyang, et al.cells were filtered through a 30 µm strainer. Dead cells were subsequently depleted using the MACS Dead Cell Removal Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). The cDNA sequencing libraries were constructed using

(20) RASA2 deletion rescues immune synapse dysfunction, enhancing CAR T cell efficacy against DMGsJournal for ImmunoTherapy of CancerMarch 30, 2026Jorge Ibanez-Vega, Robert Teis, Jennifer K Ocasio, Peter Chockley, Aamir Ansari, Alejandro Allo Anido, Sanya Mehta, Michaela Meehl, Brooke Prinzing, Meghan Ward, et al.min at room temperature, and cells were filtered by using Miltenyi dead cell removal kit (Miltenyi; 130-090-101). Highly viable cells were recovered and prepared using the Fixation Kit (PARSE Bioscience) according

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(1)

7/13/2020Kit Works Well at Removing Dead Cells Rebecca GuyerOur lab extracts cells from human tissues for downstream analysis. Due to our processing, many dead cells are in our single cell suspension and we need to remove as many as possible before doing any ... Read Review
(1)

6/4/2018Dead Cell Removal Kit Alex RayOur lab studies HIV immunology and the enrichment of live cells for downstream analysis is important for many of our protocols. This kit provides a reliable way to remove dead cells while maintaining ... Read Review
(1)

3/8/2018Dead Cell Removal Kit Lana JagannathanOur lab studies ways to enhance immune responses against HIV infection. As such we perform a lot of in vitro killing assays with different effector cells against HIV infected PBMC. One way to measure ... Read Review
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7/8/2016Dead Cell Separation from Single Cell Preparation Sivaraman NatarajanWe prepare single cells from various tissues for our Genomic studies. We encountered lot of damaged and dead cells in the end from the preparation. This kit works works on MACS ... Read Review

Dead Cell Removal Kit (up to 100 separations)

Dead Cell Removal Kit from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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Dead Cell Removal Kit from Miltenyi Biotec

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