V450 Mouse Anti-Human CD45 Clone 2D1 Flow Cytometry Staining

June 20, 2016

BlueskyReddit
Inflammation & Ageing
University of Birmingham
PhD Student

Overall

Quality of Results

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Company:

BD Biosciences

Product Name:

V450 Mouse Anti-Human CD45 Clone 2D1 (RUO (GMP))

Catalog Number:

642275

Human synovial cells yielded by digestion were stained with anti-human CD45 to identify cells of a haematopoeitic origin.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human synovial cells

Primary Incubation

30 minute incubation at 4oC. Live/dead stain.

Blocking Agent

No specific blocking step. 2% FCS in staining buffer.

Secondary Incubation

30 minute incubation at 4oC. Antibody cocktail used at this point containing antibody at 2ug/ml.

Tertiary Incubation

None

Detection

Mo Flow Astrios EQ 405 excitation448/59 bandpass filter for emission

Results Summary

Antibody worked well and gave a clear CD45 positive population. Additionally it can be used at low concentration compared to the volume supplied

Additional Notes

None

Related Categories

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Summary

The Good

Antibody worked well and produced clear positive population

The Bad

No particular bad points

The Bottom Line

Very good antibody with decent intensity of staining and a large amount supplied.

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