Time Course Effect of Chemoattractant on CSN3 Expression

June 17, 2016

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz

Product Name:

CSN3

Catalog Number:

sc-100693

Casein Kappa or CSN3 belongs to COP9 signalosome family which has wide range of cellular applications in proteosomal degradation. Here we checked the time course effect of CSN3 by chemoattractant.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

Mouse cell lysate

Primary Incubation

1:1000 in 5% BSA prepared in 1x TBST

Blocking Agent

5% milk prepared in 1x TBST

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

NA

Detection

ECL

Results Summary

We found there is no increase in CSN3 expression by chemoattractant.

Additional Notes

None

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Summary

The Good

This antibody is very good for immunoprecipitation and western blot

The Bad

None

The Bottom Line

This antibody works great for immunoprecipitation and western blot. Highly recommended.

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